3/21/2023 0 Comments E. vimr phagePhages recognize host bacteria by interactions between host-encoded receptors and phage-encoded receptor-binding proteins (RBPs) ( 3). Therefore, it is critical to study the mechanisms by which phages interact with their host receptors ( 2). The resistance is often related to the fact that the phage cannot recognize the host bacteria due to the mutation of receptors on the surface of the host cell. One of the biggest problems with phage therapy is that bacteria can quickly become resistant to phages. Due to the increasing drug resistance of bacteria, phage therapy is now experiencing a renaissance ( 1). The total number of global phages is estimated at roughly 10 31 phage particles, making them the most abundant microorganisms in the biosphere. Phages are natural viral bacterial predators. This model provides insight into the molecular mechanisms between K1-specific phages and their host bacteria. ![]() coli DE058: the phage PNJ1809-36 tail protein ORF261 recognizes and adsorbs to the K1 capsule, and then the K1 capsule is partially degraded, exposing the active site of LPS which is recognized by phage PNJ1809-36. Given these findings, we propose a model for the recognition process of phage PNJ1809-36 on E. Through antibody blocking assay, fluorescence labeling and high-performance gel permeation chromatography, the tail protein ORF261 of phage PNJ1809-36 was identified as the receptor-binding protein on CPS. The penultimate galactose in the outer core was identified as the specific binding region on LPS. coli DE058 and that LPS was a secondary receptor for the irreversible binding of the phage. ![]() Gene deletion, lysis curve determination, plaque formation test, adsorption assay, and inhibition assay of phage by lipopolysaccharide (LPS) showed that capsular polysaccharide (CPS) was the first receptor for the initial adsorption of PNJ1809-36 to E. A transposon mutation library was used to screen for receptor-related genes. ![]() In this study, the interactions between PNJ1809-36, a new K1-specific phage, and its host bacterium, E. K1 capsule-specific phages of Escherichia coli have been reported in recent years, but the molecular mechanism involved in host recognition of these phages remains unknown.
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